RH Logo
Hubs
About
Live
Leaderboard

Most Reputable Users

Author Profile Avatar
kusuma .
3198
Author Profile Avatar
Patrick Joyce
5786
Author Profile Avatar
Kayla Burris
771
Author Profile Avatar
Jordan Nelson
272
Author Profile Avatar
Arye Lipman
336
Author Profile Avatar
Garrett Young
195
Author Profile Avatar
xue pan
251
Author Profile Avatar
Mauz Hussain
129
Author Profile Avatar
Daniel Warner
129
Author Profile Avatar
Cullyn Newman
296

Trending Papers in biochemistry

Trending
Today
2
Authors: Bojar, Daniel, et al
Published: Jan 2014
Authors: Bojar, Daniel, et al
Published: Jan 2014
Brassinosteroids, which control plant growth and development, are sensed by the membrane receptor kinase BRASSINOSTEROID INSENSITIVE 1 (BRI1). Brassinosteroid binding to the BRI1 leucine-rich repeat (LRR) domain induces heteromerisation with a SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK)-family co-receptor. This process allows the cytoplasmic kinase domains of BRI1 and SERK to interact, trans-phosphorylate and activate each other. Here we report crystal structures of the BRI1 kinase domain in its activated form and in complex with nucleotides. BRI1 has structural features reminiscent of both serine/threonine and tyrosine kinases, providing insight into the evolution of dual-specificity kinases in plants. Phosphorylation of Thr1039, Ser1042 and Ser1044 causes formation of a catalytically competent activation loop. Mapping previously identified serine/threonine and tyrosine phosphorylation sites onto the structure, we analyse their contribution to brassinosteroid signaling. The location of known genetic missense alleles provide detailed insight into the BRI1 kinase mechanism, while our analyses are inconsistent with a previously reported guanylate cyclase activity. We identify a protein interaction surface on the C-terminal lobe of the kinase and demonstrate that the isolated BRI1, SERK2 and SERK3 cytoplasmic segments form homodimers in solution and have a weak tendency to heteromerise. We propose a model in which heterodimerisation of the BRI1 and SERK ectodomains brings their cytoplasmic kinase domains in a catalytically competent arrangement, an interaction that can be modulated by the BRI1 inhibitor protein BKI1.
Author Profile Avatar
Daniel Bojar
Author Profile Avatar
Daniel Bojar
1
Authors: Nakabayashi, Ryo, et al
Published: Jan 2021
Authors: Nakabayashi, Ryo, et al
Published: Jan 2021
Plants release specialized (secondary) metabolites from their roots to communicate with other organisms, including soil microorganisms. The spatial behavior of such metabolites around these roots can help us understand roles for the communication; however, currently they are unclear because soil-based studies are complex. Here, we established a multi-metabolomics approach using imaging mass spectrometry (IMS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to spatially assign metabolites under laboratory conditions using agar. In a case study using , we showed that 58 nitrogen (N)-containing metabolites are released from the roots into the agar. For the metabolite assignment, we used N-labeled and nonlabeled LC-MS/MS data, previously reported. Four metabolite ions were identified using authentic standard compounds as derived from monoterpene indole alkaloids (MIAs) such as ajmalicine, catharanthine, serpentine, and yohimbine. An alkaloid network analysis using dot products and spinglass methods characterized five clusters to which the 58 ions belong. The analysis clustered ions from the indolic skeleton-type MIAs to a cluster, suggesting that other communities may represent distinct metabolite groups. For future chemical assignments of the serpentine community, key fragmentation patterns were characterized using the N-labeled and nonlabeled MS/MS spectra.
Author Profile Avatar
Ayodeji Olubuse
Slide 1 of 1
  • Paper Preview Page 1
Author Profile Avatar
Ayodeji Olubuse
1
Authors: Zhang, Xin, et al
Published: Jan 2021
Authors: Zhang, Xin, et al
Published: Jan 2021
The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism and appetite, and is an important target for treatment of type II diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials, such as semaglutide, progressing to approval. Others, including taspoglutide, failed through high incidence of side-effects or insufficient efficacy. GLP-1R agonists have a broad spectrum of signalling profiles. However, molecular understanding is limited by a lack of structural information on how different GLP-1R agonists engage with the GLP-1R. In this study, we determined cryo-electron microscopy (cryo-EM) structures of GLP-1R-Gs protein complexes bound with semaglutide and taspoglutide. These revealed similar peptide binding modes to that previously observed for GLP-1. However, 3D variability analysis of the cryo-EM micrographs revealed different motions within the bound peptides and the receptor relative to when GLP-1 is bound. This work provides novel insights into the molecular determinants of peptide engagement with the GLP-1R.
Author Profile Avatar
Ayodeji Olubuse
Author Profile Avatar
Ayodeji Olubuse
1
From Paper: Chemical synthesis and purification of a non-hydrolyzable CTP analog CTPγS
Authors: M Rejzek, Tung B. K. Le
Published: Jan 2021
From Paper: Chemical synthesis and purification of a non-hydrolyzable CTP analog CTPγS
Authors: M Rejzek, Tung B. K. Le
Published: Jan 2021
  • A classical approach to the chemical synthesis of γ-thiophosphate analogs of nucleoside triphosphates is based on S-2-carbamoylethyl thiophosphate activation by diphenyl phosphorochloridate (Goody and Eckstein, 1971). The activated reagent was used to phosphorylate nucleoside diphosphates.
Author Profile Avatar
Ayodeji Olubuse
Author Profile Avatar
Ayodeji Olubuse
1
Authors: Fernández-Rodríguez, Carmen, et al
Published: Jan 2021
Authors: Fernández-Rodríguez, Carmen, et al
Published: Jan 2021
Cystathionine β-synthase (CBS), the pivotal enzyme of the reverse transsulfuration pathway, catalyzes the pyridoxal-5’-phosphate-dependent condensation of serine with homocysteine to form cystathionine. Additionally, CBS performs alternative reactions that use homocysteine and cysteine as substrates leading to the endogenous biosynthesis of hydrogen sulfide (H S), an important signal transducer in many physiological and pathological processes. the causative agent of toxoplasmosis, encodes a functional CBS ( CBS) that contrary to human CBS, is not allosterically regulated by S-adenosylmethionine and can use both, Ser and -acetylserine (OAS) as substrates. CBS is also strongly implicated in the production of H S, and thus involved in redox homeostasis of the parasite. Here, we report its crystal structure, the first CBS from a protozoan described so far. Our data reveals a basal-like fold that unexpectedly differs from the active conformations found in other organisms, but structurally similar to the pathogenic activated mutant D444N of the human enzyme.
Author Profile Avatar
Ayodeji Olubuse
Slide 1 of 1
  • Paper Preview Page 1
Author Profile Avatar
Ayodeji Olubuse
1
From Paper: Characterisation and chemometric evaluation of 17 elements in ten seaweed species from Greenland
Authors: Kreissig, Katharina J., et al
Published: Jan 2021
From Paper: Characterisation and chemometric evaluation of 17 elements in ten seaweed species from Greenland
Authors: Kreissig, Katharina J., et al
Published: Jan 2021
  • Several Greenland seaweed species have potential as foods or food ingredients, both for local consumption and export. However, knowledge regarding their content of beneficial and deleterious elements on a species specific and geographical basis is lacking.
Author Profile Avatar
Ayodeji Olubuse
Slide 1 of 1
  • Paper Preview Page 1
Author Profile Avatar
Ayodeji Olubuse
238
Published: Nov 2020
Published: Nov 2020
  • The results indicate that mothers fed on a low protein diet during gestation and lactation produce male offspring with normal sperm morphology, concentration and motility but exhibiting an overall decrease of fertility when they reach adulthood.
  • This study used a mouse model to investigate the effects of maternal undernutrition on fertility in male progeny
Author Profile Avatar
Kayla Burris
Slide 1 of 1
  • Paper Preview Page 1
232
Published: Oct 2016
Published: Oct 2016
  • Application of this approach to other cancers with less standard treatments will introduce heterogeneity into clinical outcome and would need to be accounted for in the statistical analyses
  • In this manuscript the authors present a bioinformatics approach for connectivity mapping based on clinical outcomes collected on a large patient population followed by functional validation of the identified drugs
Author Profile Avatar
Chen Wang
Slide 1 of 1
  • Paper Preview Page 1
2
Published: Feb 2021
Published: Feb 2021
Although the use of computational methods within the pharmaceutical industry is well established, there is an urgent need for new approaches that can …
Author Profile Avatar
ALYSSA ENG
Load More Papers