Attempts were made to develop RP-HPLC method for simultaneous estimation of Itopride HCl & Pantoprazole for the RP - HPLC method, Waters Gradient System with UV Detector and C18 column with 250mm x 4.6 mm i.d and 5μm particle size. After different trial run injections with different solvent mixture ratio and pH of the mobile phase is also alter during one trail condition, From all the traial injection separation of peaks are observed in the mobile phase of the Acetonitrile: 0.05 % OPA (60:40) ration hence this mobile phase ration and pH was used as the mobile phase for the method. The detection wavelength was 252 nm and flow rate was 0.7 ml/min. In the developed method, the retention time of Itopride HCl and Pantoprazole were found to be 4.42 min and 8.59 min. The developed method can be used for the routine quality control analysis of Itopride HCl and Pantoprazole in bulk drug as well as in formulations after complete method Validation.