Abstract

Several methods exist for constructing sequencing libraries from single-cell amounts of RNA. However, different library preparation methods are required to enable full length or strand specific 3 end profiling. The Integrated Cell Expression Toolkit (ICE-T) maximizes functionality by integrating these approaches with novel strategies that provide a range of profiling options.\n\nTranscriptome profiling at high cellular resolution has many applications in biology but is hampered by a need to boost the representation of RNA to guard against losses during processing and enable efficient use of high throughput technologies. RNA amplification techniques developed for profiling limiting amounts of RNA by microarray have recently been adapted to NGS1-5. These various, but distinct, techniques enable both full length profiling3 and strand specific 3 end profiling2, 4 ...