Cannabis sativa has long been an important source of fiber extracted from hemp and both medicinal and recreational drugs based on cannabinoid compounds. Here, we investigated its poorly known domestication history using whole-genome resequencing of 110 accessions from worldwide origins. We show that C. sativa was first domesticated in early Neolithic times in East Asia and that all current hemp and drug cultivars diverged from an ancestral gene pool currently represented by feral plants and landraces in China. We identified candidate genes associated with traits differentiating hemp and drug cultivars, including branching pattern and cellulose/lignin biosynthesis. We also found evidence for loss of function of genes involved in the synthesis of the two major biochemically competing cannabinoids during selection for increased fiber production or psychoactive properties. Our results provide a unique global view of the domestication of C. sativa and offer valuable genomic resources for ongoing functional and molecular breeding research. Genome analyses provide new insights into the global domestication history of Cannabis sativa and its two main cannabinoids. Genome analyses provide new insights into the global domestication history of Cannabis sativa and its two main cannabinoids.
This article considers the interdisciplinary opportunities and challenges of working with digital cultural heritage, such as digitized historical newspapers, and proposes an integrated digital hermeneutics workflow to combine purely disciplinary research approaches from computer science, humanities, and library work. Common interests and motivations of the above-mentioned disciplines have resulted in interdisciplinary projects and collaborations such as the NewsEye project, which is working on novel solutions on how digital heritage data is (re)searched, accessed, used, and analyzed. We argue that collaborations of different disciplines can benefit from a good understanding of the workflows and traditions of each of the disciplines involved but must find integrated approaches to successfully exploit the full potential of digitized sources. The paper is furthermore providing an insight into digital tools, methods, and hermeneutics in action, showing that integrated interdisciplinary research needs to build something in between the disciplines while respecting and understanding each other's expertise and expectations.
Modern humans appeared in Europe by at least 45,000 years ago1–5, but the extent of their interactions with Neanderthals, who disappeared by about 40,000 years ago6, and their relationship to the broader expansion of modern humans outside Africa are poorly understood. Here we present genome-wide data from three individuals dated to between 45,930 and 42,580 years ago from Bacho Kiro Cave, Bulgaria1,2. They are the earliest Late Pleistocene modern humans known to have been recovered in Europe so far, and were found in association with an Initial Upper Palaeolithic artefact assemblage. Unlike two previously studied individuals of similar ages from Romania7 and Siberia8 who did not contribute detectably to later populations, these individuals are more closely related to present-day and ancient populations in East Asia and the Americas than to later west Eurasian populations. This indicates that they belonged to a modern human migration into Europe that was not previously known from the genetic record, and provides evidence that there was at least some continuity between the earliest modern humans in Europe and later people in Eurasia. Moreover, we find that all three individuals had Neanderthal ancestors a few generations back in their family history, confirming that the first European modern humans mixed with Neanderthals and suggesting that such mixing could have been common.