Pretreatment of animals with the synthetic DNA-encoded arginase led to significant decreases in skin acanthosis, proinflammatory cytokines, and costimulatory molecules in extracted macrophages and dendritic cells
Significant in vivo immunosuppression was observed in the presence of DNA-encoded arginase
The authors demonstrate Boolean logic using their CHOMP circuits
Viral proteases are highly specific for short cognate sites and there is wide natural diversity - the authors focused on tobacco etch virus protease (TEVP) and related proteases to build a complex protein circuit in HEK293 cells
This paper reviews this state of the art in measuring plant signaling, using principles and tools borrowed from and inspired by engineering, as well as efforts to use this knowledge to enable rapid, rational re-engineering of plant development.
Sensitive, genetically encoded reporters (biosensors), in combination with emerging single-cell transcriptomics approaches, are providing increasingly detailed molecular descriptions of cells undergoing developmental transitions
Computational simulation of peptide adsorption at the aqueous gold interface is key to advancing the development of many applications based on gold nanoparticles, ranging from nanomedical devices to smart biomimetic materials. Here, we present a force field, GolPCHARMM, designed to capture peptide adsorption at both the aqueous Au(111) and Au(100) interfaces. The force field,compatible with the bio-organic force field CHARMM, is parametrized using a combination of experimental and firstprinciples data. Like its predecessor, GolP (Iori, F.; et al. J. Comput. Chem. 2009, 30, 1465), this force field contains terms to describe the dynamic polarization of gold atoms, chemisorbing species, and the interaction between sp2 hybridized carbon atoms and gold. A systematic study of small molecule adsorption at both surfaces using the vdW-DF functional (Dion, M.; et al. Phys. Rev. Lett. 2004, 92, 246401−1. Thonhauser, T.; et al. Phys. Rev. B 2007, 76, 125112) is carried out to fit and test force field parameters and also, for the first time, gives unique insights into facet selectivity of gold binding in vacuo. Energetic and spatial trends observed in our DFT calculations are reproduced by the force field under the same conditions. Finally, we use the new force field to calculate adsorption energies, under aqueous conditions, for a representative set of amino acids. These data are found to agree with experimental findings.