In 2020 synthetic biology turned 20 years old. It’s first decade saw some impressive research papers, lots of visionary thinking and unprecedented excitement, but its second decade—from 2010 to 2020—was when the hype really needed to be replaced by some real achievements. So how has it done?
Pretreatment of animals with the synthetic DNA-encoded arginase led to significant decreases in skin acanthosis, proinflammatory cytokines, and costimulatory molecules in extracted macrophages and dendritic cells
Significant in vivo immunosuppression was observed in the presence of DNA-encoded arginase
The authors demonstrate Boolean logic using their CHOMP circuits
Viral proteases are highly specific for short cognate sites and there is wide natural diversity - the authors focused on tobacco etch virus protease (TEVP) and related proteases to build a complex protein circuit in HEK293 cells
This paper reviews this state of the art in measuring plant signaling, using principles and tools borrowed from and inspired by engineering, as well as efforts to use this knowledge to enable rapid, rational re-engineering of plant development.
Sensitive, genetically encoded reporters (biosensors), in combination with emerging single-cell transcriptomics approaches, are providing increasingly detailed molecular descriptions of cells undergoing developmental transitions